Publication
Title
Non-equilibrium dielectric barrier discharge treatment of mesenchymal stem cells : charges and reactive oxygen species play the major role in cell death
Author
Abstract
Atmospheric pressure non‐equilibrium plasmas are efficacious in killing both prokaryotic and eukaryotic cells. While the mechanism of plasma induced cell death has been thoroughly studied in prokaryotes, detailed investigation of plasma mediated eukaryotic cell death is still pending. When plasma is generated, four major components that interact with cells are produced: electric fields, radiation, charged particles, and neutral gas species. The goal of this study was to determine which of the plasma components are responsible for plasma‐induced cell death by isolating and removing each from treatment. The C3H10T1/2 murine mesenchyme stem cell line was treated in six well plates, stained with Propidium Iodide to determine viability, and analyzed by image cytometry. Our results show that plasma‐generated charges and reactive oxygen species are the primary contributors to cell death.
Language
English
Source (journal)
Plasma processes and polymers. - Weinheim
Plasma processes and polymers. - Weinheim
Publication
Weinheim : 2015
ISSN
1612-8850
Volume/pages
12 :10 (2015) , p. 1117-1127
ISI
000363731700009
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 12.12.2018
Last edited 26.09.2021