Publication
Title
Enzymatic sensor for phenols based on titanium dioxide generating surface confined ROS after treatment with $H_{2}O_{2}$
Author
Abstract
 Titanium dioxide (TiO2) is a popular material as host matrix for enzymes. We now evidence that TiO2 can accumulate and retain reactive oxygen species after treatment by hydrogen peroxide (H2O2) and support redox cycling of a phenolic analyte between horseradish peroxidase (HRP) and an electrode. The proposed detection scheme is identical to that of second generation biosensors, but the measuring solution requires no dissolved H2O2. This significantly simplifies the analysis and overcomes issues related to H2O2 being present (or generated) in the solution. The modified electrodes showed rapid stabilization of the baseline, a low noise level, fast realization of a steady-state current response, and, in addition, improved sensitivity and limit of detection compared to the conventional approach, i.e. in the presence of H2O2 in the measuring solution. Hydroquinone, 4-aminophenol, and other phenolic compounds were successfully detected at sub-μM concentrations. Particularly, a linear response in the concentration range between 0.025 and 2 μM and LOD of 24 nM was demonstrated for 4-aminophenol. The proposed sensor design goes beyond the traditional concept with three sensors generations offering a new possibility for the development of enzymatic sensors based on peroxidases and the formation of ROS on titania after treatment with H2O2.
Language
English
Source (journal)
Sensors and actuators : B : chemical. - Lausanne
Publication
Lausanne : 2019
ISSN
0925-4005
Volume/pages
283 (2019) , p. 343-348
ISI
000455854000043
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
 Faculty/Department Research group Project info Highly visible light responsive black titania for photo-electrochemical applications: the electrosensing of polyphenols in flow mode.Towards new approaches in bioelectrochemistry – Targeted immobilization of globins on porous materials. Publication type Subject Affiliation Publications with a UAntwerp address
External links
 Web of Science
Record
 Identification Creation 12.12.2018 Last edited 04.09.2021 To cite this reference