Publication
Title
Human papillomavirus detection in urine : effect of a first-void urine collection device and timing of collection
Author
Abstract
Great interest has been directed towards the use of first-void (FV) urine as a liquid biopsy for high-risk HPV DNA testing. The aim of this study was to investigate the potential effect of a first generation FV urine collection device on the detection of HPV DNA and to assess if the concentration of HPV DNA varies between FV urine collected in the morning and those collected later during the day. In this prospective cohort study, 33 self-reported HPV-positive women participated. An FV urine sample was collected by these women in the morning (first urine of the day) and another sample was collected later that day for four consecutive days using two different collection methods; i.e., the Colli-Pee (R) and a standard urine cup. Samples were collected at home and returned at ambient temperature to the laboratory by postal mail. HPV DNA testing was conducted with the Riatol qPCR HPV genotyping assay. Based on the combined generalized linear mixed model used, there was no significant impact of the timing of collection (morning versus later during the day) on copies of HPV DNA, whereas Colli-Pee (R) collected samples show higher HPV concentrations than cup collected samples. However, at high concentrations of hDNA, the benefit of the Colli-Pee (R) disappeared.
Language
English
Source (journal)
Journal of virological methods. - Amsterdam
Publication
Amsterdam : 2019
ISSN
0166-0934
DOI
10.1016/J.JVIROMET.2018.11.008
Volume/pages
264 (2019) , p. 23-30
ISI
000456222300004
Pubmed ID
30452931
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 06.02.2019
Last edited 02.10.2024
To cite this reference