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Title
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Evidence for viable and stable triploid Trypanosoma congolense parasites
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Author
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Abstract
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| Background Recent whole genome sequencing (WGS) analysis identified a viable triploid strain of Trypanosoma congolense. This triploid strain BANANCL2 was a clone of the field isolate BANAN/83/CRTRA/64 that was collected from cattle in Burkina Faso in 1983. Results We demonstrated the viability and stability of triploidy throughout the complete life-cycle of the parasite by infecting tsetse flies with the triploid clone BANANCL2. Proboscis-positive tsetse flies efficiently transmitted the parasites to mice resulting in systemic infections. WGS of the parasites was performed at all life-cycle stages, and a method based on a block alternative allele frequency spectrum was developed to efficiently detect the ploidy profiles of samples with low read depth. This approach confirmed the triploid profile of parasites throughout their life-cycle in the tsetse fly and the mammalian host, demonstrating that triploidy is present at all stages and is stable over time. Conclusion The presence of viable field-isolated triploid parasites indicates another possible layer of genetic diversity in natural T. congolense populations. The comparison between triploid and diploid parasites provides a unique model system to study the impact of chromosome copy number variations in African trypanosomes. In addition, the consequences of triploidy can be further investigated using this stable triploid model. |
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Language
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English
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Source (journal)
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Parasites and vectors
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Publication
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2017
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ISSN
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1756-3305
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DOI
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10.1186/S13071-017-2406-Z
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Volume/pages
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10
(2017)
, 8 p.
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Article Reference
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468
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ISI
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000412693600011
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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