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Title
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Thyroid hormone disruptors interfere with molecular pathways of eye development and function in zebrafish
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Author
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Abstract
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| The effects of thyroid hormone disrupting chemicals (THDCs) on eye development of zebrafish were investigated. We expected THDC exposure to cause transcriptional changes of vision-related genes, which find their phenotypic anchoring in eye malformations and dysfunction, as observed in our previous studies. Zebrafish were exposed from 0 to 5 days post fertilization (dpf) to either propylthiouracil (PTU), a thyroid hormone synthesis inhibitor, or tetrabromobisphenol-A (TBBPA), which interacts with thyroid hormone receptors. Full genome microarray analyses of RNA isolated from eye tissue revealed that the number of affected transcripts was substantially higher in PTU- than in TBBPA-treated larvae. However, multiple components of phototransduction (e.g., phosphodiesterase, opsins) were responsive to both THDC exposures. Yet, the response pattern for the gene ontology (GO)-class sensory perception differed between treatments, with over 90% down-regulation in PTU-exposed fish, compared to over 80% up-regulation in TBBPA-exposed fish. Additionally, the reversibility of effects after recovery in clean water for three days was investigated. Transcriptional patterns in the eyes were still altered and partly overlapped between 5 and 8 dpf, showing that no full recovery occurred within the time period investigated. However, pathways involved in repair mechanisms were significantly upregulated, which indicates activation of regeneration processes. |
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Language
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English
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Source (journal)
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International journal of molecular sciences
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Publication
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2019
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ISSN
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1422-0067
1661-6596
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DOI
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10.3390/IJMS20071543
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Volume/pages
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20
:7
(2019)
, 17 p.
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Article Reference
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1543
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ISI
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000464980400010
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Pubmed ID
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30934780
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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