Title
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Real-time PCR melting analysis with fiber optic SPR enables multiplex DNA identification of bacteria
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Author
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Abstract
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A fiber optic surface plasmon resonance (FO-SPR) technology was developed that enables simultaneous quantification and identification of multiple DNA targets on the same platform. The bioassay was based on the hybridization/melting of DNA-coated Au nanoparticles on the FO-SPR sensor when targets are present. The multiplex concept was successfully demonstrated on two related bacteria and for detection of multiple mutations in sequences. In conclusion, FO-SPR technology shows a great potential as a next generation in vitro diagnostics tool. |
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Language
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English
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Source (journal)
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The analyst. - Cambridge, 1876, currens
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Publication
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Cambridge
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2016
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ISSN
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0003-2654
[print]
1364-5528
[online]
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DOI
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10.1039/C5AN02342D
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Volume/pages
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141
:6
(2016)
, p. 1906-1911
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ISI
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000372259100004
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Full text (Publisher's DOI)
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