Publication
Title
Real-time PCR melting analysis with fiber optic SPR enables multiplex DNA identification of bacteria
Author
Abstract
A fiber optic surface plasmon resonance (FO-SPR) technology was developed that enables simultaneous quantification and identification of multiple DNA targets on the same platform. The bioassay was based on the hybridization/melting of DNA-coated Au nanoparticles on the FO-SPR sensor when targets are present. The multiplex concept was successfully demonstrated on two related bacteria and for detection of multiple mutations in sequences. In conclusion, FO-SPR technology shows a great potential as a next generation in vitro diagnostics tool.
Language
English
Source (journal)
The analyst. - Cambridge, 1876, currens
Publication
Cambridge : 2016
ISSN
0003-2654 [print]
1364-5528 [online]
DOI
10.1039/C5AN02342D
Volume/pages
141 :6 (2016) , p. 1906-1911
ISI
000372259100004
Full text (Publisher's DOI)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identifier
Creation 20.06.2019
Last edited 05.12.2024
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