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Title
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Biotransformation to produce the anticancer compound colchicoside using cell suspension cultures of Astragalus vesicarius plant species
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Author
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Abstract
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| This paper discusses the biotechnological process affected by means of plant suspension cultures, for production of colchicoside, the 3-O-glucosyl derivative of 3-O-demethylcolchicine. Colchicoside can be considered as an antitumoural prodrug which is activated after oral administration and may have more beneficial effects and a better toxicity profile (because of a slow-release effect) than colchicine. We have developed a green and efficient biotechnological method using colchicine, as a precursor, derived from its natural source G. superba seeds. Plant suspension cultures of Astragalus vesicarius were used to design a practical biotechnological platform to replace a methyl group at C-3 regiospecifically by a glycosyl moiety in colchicine. Using different concentrations of a colchicine-rich extract, the maximum enzymatic potential of Astragalus vesicarius suspension cells was achieved. According to quantitative HPLC-UV analysis, levels of 9.35 mol/g DW colchicoside were achieved. This is the first report of region-specific glycosylation at C-3 of the aromatic ring A of the colchicine using plant suspension cultures. |
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Language
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English
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Source (journal)
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NATURAL PRODUCT COMMUNICATIONS
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Publication
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2019
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ISSN
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1934-578X
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Volume/pages
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14
:1
(2019)
, p. 27-29
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ISI
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000469340700008
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Full text (open access)
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Full text (publisher's version - intranet only)
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