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Title
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Scavenger receptor-mediated endocytosis facilitates RNA interference in the desert locust, Schistocerca gregaria
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Author
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Abstract
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| RNA interference (RNAi) has become a widely used loss-of-function tool in eukaryotes; however, the delivery of double-stranded (ds)RNA) to the target cells remains a major challenge when exploiting the RNAi-technology. In insects, the efficiency of RNAi is highly species-dependent. Yet, the mechanism of cell entry in insects has only been characterized in a cell line of the fruit fly, Drosophila melanogaster, a species that is well known to be poorly amenable to environmental RNAi. In the present paper, we demonstrate that silencing vacuolar H-ATPase 16 (vha16) and clathrin heavy chain (clath), two components of the Clathrin-dependent endocytosis pathway, together with pharmacological inhibition of scavenger receptors with polyinosine and dextran sulphate, can significantly attenuate the highly robust RNAi response in the desert locust, Schistocerca gregaria. |
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Language
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English
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Source (journal)
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Insect molecular biology. - Oxford
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Publication
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Oxford
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2014
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ISSN
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0962-1075
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DOI
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10.1111/IMB.12083
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Volume/pages
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23
:3
(2014)
, p. 320-329
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ISI
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000335577400004
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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