Functional Characterization of the Short Neuropeptide F Receptor in the Desert Locust, Schistocerca gregaria

Dillen, Senne; Zels, Sven; Verlinden, Heleen; Spit, Jornt; Van Wielendaele, Pieter; Vanden Broeck, Jozef

doi:10.1371/JOURNAL.PONE.0053604

Title

Functional Characterization of the Short Neuropeptide F Receptor in the Desert Locust, Schistocerca gregaria

Author

Dillen, Senne

Zels, Sven

Verlinden, Heleen

Spit, Jornt

Van Wielendaele, Pieter

Vanden Broeck, Jozef

Abstract

Whereas short neuropeptide F (sNPF) has already been reported to stimulate feeding behaviour in a variety of insect species, the opposite effect was observed in the desert locust. In the present study, we cloned a G protein-coupled receptor (GPCR) cDNA from the desert locust, Schistocerca gregaria. Cell-based functional analysis of this receptor indicated that it is activated by both known isoforms of Schgr-sNPF in a concentration dependent manner, with EC50 values in the nanomolar range. This Schgr-sNPF receptor constitutes the first functionally characterized peptide GPCR in locusts. The in vivo effects of the sNPF signalling pathway on the regulation of feeding in locusts were further studied by knocking down the newly identified Schgr-sNPF receptor by means of RNA interference, as well as by means of peptide injection studies. While injection of sNPF caused an inhibitory effect on food uptake in the desert locust, knocking down the corresponding peptide receptor resulted in an increase of total food uptake when compared to control animals. This is the first comprehensive study in which a clearly negative correlation is described between the sNPF signalling pathway and feeding, prompting a reconsideration of the diverse roles of sNPFs in the physiology of insects.

Language

English

Source (journal)

PLoS ONE

Publication

2013

ISSN

1932-6203

DOI

10.1371/JOURNAL.PONE.0053604

Volume/pages

8 :1 (2013) , 9 p.