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Title
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First-void urine as a non-invasive liquid biopsy source to detect vaccine-induced human papillomavirus antibodies originating from cervicovaginal secretions
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Author
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Abstract
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| Background: Monitoring HPV antibodies non-invasively would be a major advantage for large epidemiological studies and follow-up of vaccinees. Objectives: This study investigated the presence of HPV-specific antibody transudates from systemic circulation in first-void urine of (un)vaccinated subjects and the agreement with paired sera. Study design: In this case-control study, 55 paired first-void urine and serum samples were included from 19- to 26-year-old women, unvaccinated (n = 19) or vaccinated (n = 36) with the bi- or quadrivalent HPV vaccine during adolescence (NCT02714114). Human IgA, total human IgG, and HPV6/11/16/18-Ig(M/G/A) were measured in paired samples. Results: Significant positive Spearman rank correlations (r(s)) were found in HPV-specific antibody levels between paired samples (HPV6: r(s) = 0.777; HPV11: r(s) = 0.757; HPV16: r(s) = 0.876; HPV18: r(s) = 0.636 (p < 0.001)). In both first-void urine and serum, significantly higher HPV6/11/16/18 antibody levels were observed in vaccinated compared with unvaccinated women (p <= 0.017). Conclusions: The present study provides the first proof that vaccine-induced HPV antibodies are detectable in the first-void urine of young women. Moreover, significant positive correlations were observed between HPV6/11/16/18-antibodies in first-void urine and paired sera. Further optimization and validation are required to demonstrate its potential use in epidemiological studies and follow-up of HPV vaccination. |
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Language
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English
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Source (journal)
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Journal of clinical virology. - Rome
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Publication
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Rome
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2019
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ISSN
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1386-6532
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DOI
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10.1016/J.JCV.2019.05.004
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Volume/pages
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117
(2019)
, p. 11-18
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ISI
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000475867400003
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Pubmed ID
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31129514
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Full text (Publisher's DOI)
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Full text (open access)
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