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Title
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Migrating a lab-developed MERS-CoV real-time PCR to 3 "Sample to Result" systems: experiences on optimization and validation
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Author
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Abstract
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| The goal of the study was to adapt our Middle East respiratory syndrome coronavirus (MERS-CoV) lab-developed test (LDT) to 3 "Sample to Result" (S2R) systems: BD MAX (BD), ELITe InGenius (ELITechGroup), and ARIES (Luminex). The BD MAX and InGenius system allowed use of lab-developed primers and TaqMan probes, while ARIES required conversion to MultiCode primers for melting curve analysis. Each device required <= 1 day of training and assay optimization. No discordant results were noted after analysis of 32 External Quality Control (EQC) samples. On a 10-fold dilution series of a MERS-CoV-positive EQC sample, InGenius obtained the highest detection rate. Laboratory technicians rated the ARIES as the user-friendliest. It also required the least hands-on time. BD MAX had the lowest turnaround time and highest throughput. While each device had distinguishing system properties with associated (dis)advantages, the 3 S2R systems were comparable in terms of assay development and validation. (C) 2019 Elsevier Inc. All rights reserved. |
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Language
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English
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Source (journal)
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Diagnostic microbiology and infectious disease. - Amsterdam
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Publication
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Amsterdam
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2019
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ISSN
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0732-8893
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DOI
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10.1016/J.DIAGMICROBIO.2019.02.006
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Volume/pages
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94
:4
(2019)
, p. 349-354
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ISI
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000478701800008
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Pubmed ID
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30929995
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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