Publication
Title
TGF-$\beta$ and MIP-1$\alpha$ exert their main inhibitory activity on very primitive $CD34^{++}CD38^{-}$ cells but show opposite effects on more mature $CD34^{+}CD38^{+}$ human hematopoietic progenitors
Author
Abstract
 We investigated the effects of transforming growth factor-beta (TGF-beta) and macrophage inflammatory protein-1 alpha (MIP-1 alpha) on very primitive CD34(++)CD38(-) and on more mature CD34(+)CD38(+) human hematopoietic progenitor cells by means of a two stage pre-colony-forming cell (pre-CFC) assay. The first (liquid) stage of this assay allows evaluation of the effects of TGF-beta and MIP-1 alpha on the ''primary'' proliferation of the progenitors under study and on the generation of ''secondary'' colony-forming cells (CFC, cells for which a second stage semisolid clonogenic assay was used as a read-out). TGF beta inhibited the proliferation and CFC generation of CD34(++)CD38(-) and CD34(+)CD38(+) cells, showing the strongest inhibitory activity on CD34(++)CD38(-) cells. MIP-1 alpha exerted a weaker inhibitory activity on CD34(++)CD38(-) cells, whereas it enhanced the primary proliferation of CD34(+)CD38(+) cells and generation of secondary CFC in this subpopulation. Thus, TGF-beta and MIP-1 alpha both inhibit very primitive CD34(++)CD38(-) cells, but they are not equally potent. The effects of TGF-beta and MIP-1 alpha on more mature progenitor cells are more complex. Our results and data from the literature indicate that, as progenitor cells mature, they reach a ''pivotal point'' at a certain stage in their differentiation pathway, depending on the inhibitor, where they are no longer inhibited or where they may even be stimulated by the former inhibitor to proliferate.
Language
English
Source (journal)
Experimental hematology. - Oak Ridge, Tenn., 1973, currens
Publication
Oak Ridge, Tenn. : 1996
ISSN
0301-472X
1873-2399 [online]
Volume/pages
24:13(1996), p. 1509-1515
ISI
A1996VW26000008
UAntwerpen
 Faculty/Department Research group Publication type Subject Affiliation Publications with a UAntwerp address