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Title
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Colorectal anastomotic leakage : a new experimental model
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Author
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Abstract
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| Background. Anastomotic leakage is the major complication after colorectal surgery. To date, animal experiments concerning colorectal anastomosis focus on anastomotic healing instead of anastomotic leakage. This study aims to develop a new experimental model for colorectal anastomotic leakage. Methods. A control group, receiving an anastomosis with 12 interrupted sutures, was compared to a group receiving an anastomosis with 6 interrupted sutures. When the leakage rate was observed to be too low, the number of sutures was decreased stepwise, to 5 or less. Each group contained 9 "C57B16-mice". After 7 d the Anastomotic Bursting Pressure (ABP) was determined. Results. In the first experiment, one mouse (11.1%) in the case group and none in the control group developed leakage. Average ABP was 152,2 mmHg in the control group and 138,8 mmHg in the case group (P = 0,111). In the second experiment, case group receiving an anastomosis with 5 sutures, 4 mice (44.4%) in the case group developed leakage. This experiment was repeated twice resulting in leakage rates of 33.3% and 44.4%. The average overall ABP in the case group was 142,7 mmHg vs. 179,9 mmHg (P = 0,022) in the control group. The mice without leakage showed a stabilization of average weight loss around day 2 and 3 and a decrease afterwards. The mice with leakage showed a decrease only after day 5. The difference in wellness-scores between the groups with- and without leakage was 2 points, increasing during follow-up. Conclusions. The model of anastomotic leakage caused by creating an anastomosis with 5 interrupted sutures is feasible. Weight loss and wellness-scores are good predictors of leakage. |
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Language
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English
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Source (journal)
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Journal of surgical research. - Philadelphia, Pa
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Publication
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Philadelphia, Pa
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2009
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ISSN
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0022-4804
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DOI
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10.1016/J.JSS.2008.08.019
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Volume/pages
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155
:1
(2009)
, p. 7-12
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ISI
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000267448500002
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Full text (Publisher's DOI)
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