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Title
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Potential in vivo transfer of a plasmid established by combining long- and short-read sequencing
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Author
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Abstract
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| Horizontal transfer of plasmid-mediated antibiotic resistance has rarely been documented in vivo. Utilizing long-read (Oxford Nanopore) and short-read (Illumina) sequencing, we confirmed that a gut-colonizing Escherichia coli and a hypervirulent Klebsiella pneumoniae ST23, isolated from a surgical site culture of a patient receiving cefuroxime therapy, harboured a 100% identical blaCTX-M-14-harbouring plasmid, indicative of a potential transfer in vivo. |
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Language
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English
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Source (journal)
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Journal of microbiological methods. - Amsterdam
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Publication
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Amsterdam
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2019
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ISSN
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0167-7012
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DOI
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10.1016/J.MIMET.2019.02.004
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Volume/pages
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159
(2019)
, p. 1-4
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ISI
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000465050500001
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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