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Title
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Determination of Se in human serum by AAS using electrothermal atomization with longitudinal zeeman-effect background correction or flow injection hydride generation
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Author
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Abstract
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| Two methods are described for the determination of selenium in human serum by atomic absorption spectrometry (AAS) using either electrothermal atomization (ET) with longitudinal Zeeman-effect background correction or flow injection (FI) hydride generation (HG). ETAAS allowed the direct determination of selenium in an appropriately diluted serum sample (1:10). Serum selenium was stabilized prior to atomization by a matrix modifier containing 1500 mg/L Pd and 1000 mg/L Mg nitrate. Calibration was performed by the method of standard addition. FI-HGAAS required a nitric/perchloric acid digestion procedure prior to analysis. Calibration was performed using a calibration curve in 1 mol/L hydrochloric acid. Both methods provided analytical data for a reference serum sample which was in close agreement with the certified value. Precision (range 2-5%) and sensitivity (similar to 50 pg) were comparable for both methods. However, ETAAS demonstrated a lower relative limit of detection, 6.5 mu g/L, compared to FI-HGAAS with 11.8 mu g/L. Accurate determinations of low selenium levels in a small sample volume (100 mu L serum sample) was obtained with both methods. |
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Language
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English
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Source (journal)
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Atomic spectroscopy. - Norwalk, Conn.
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Atomic spectroscopy
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Publication
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Norwalk, Conn.
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1995
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ISSN
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0195-5373
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Volume/pages
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16
:6
(1995)
, p. 251-255
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ISI
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A1995TL52300005
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