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Title
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Selenium content of cows milk and its distribution in protein-fractions
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Author
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Abstract
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| The present studies were undertaken to characterize selenium distribution in cow's milk. Skim milk was found to contain 93% of the total milk selenium. Irrespective of the separation technique used, skim milk selenium was mainly found to be associated with the casein fraction (> 55%). Approximately 10%, 25% and 10% of selenium was removed from skim milk, whey and casein, respectively by dialysis (molecular cut-off of 10 kDa). Isolation of individual casein and whey proteins by ion-exchange chromatography and gel filtration revealed that selenium was mainly protein bound. K-Casein was determined to be the protein richest in selenium (550 ng/g). The selenium content of the other caseins ranged from 153 ng/g to 355 ng/g. Of all the milk proteins, beta-casein contributed most to the total skim milk selenium (33% of total Se). Seventeen percent of the total skim milk selenium was associated with whey proteins and their mean selenium concentration ranged from 130 to 270 ng/g. |
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Language
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English
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Source (journal)
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Zeitschrift für Lebensmittel-Untersuchung und -Forschung. - Berlin, 1943 - 1996
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Publication
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Berlin
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1991
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ISSN
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0044-3026
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Volume/pages
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192
:5
(1991)
, p. 422-426
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ISI
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A1991FF26000002
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