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Title
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In vitro growth inhibition assays of Leishmania spp.
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Author
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Abstract
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| In vitro growth (inhibition) assays have a dual application, either supporting the discovery of novel drugs or as a monitoring tool of drug resistance in patient isolates. From an experimental design point of view, both are quite different with regard to the infecting Leishmania species and strain, the wide variety of permissive host cells (primary cells versus cell lines), drug exposure times, detection methods and endpoint criteria. Recognizing the need for enhanced assay standardization to decrease interlaboratory variation and improve proper interpretation of results, a detailed description is given of the basic fundamental procedures and requirements for routine in vitro growth of Leishmania spp. with specific focus on the intracellular amastigote susceptibility assay. Although the described experimental procedures focus on visceral Leishmania species, the same assay principles may apply for the cutaneous species as well. |
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Language
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English
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Source (journal)
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Methods in molecular biology. - Totowa, N.J., 1984, currens
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Source (book)
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Trypanosomatids : methods and protocols
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Source (series)
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Methods in molecular biology (MIMB) ; volume 2116
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Publication
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New York, N.Y.
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Humana
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2020
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ISSN
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1064-3745
1940-6029
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ISBN
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978-1-0716-0293-5
978-1-0716-0294-2
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DOI
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10.1007/978-1-0716-0294-2_47
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Volume/pages
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p. 791-800
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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