Title
Typing of **Mycoplasma pneumoniae** by nucleic acid sequence-based amplification, NASBA® Typing of **Mycoplasma pneumoniae** by nucleic acid sequence-based amplification, NASBA®
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
London ,
Subject
Chemistry
Biology
Human medicine
Engineering sciences. Technology
Source (journal)
Molecular and cellular probes. - London
Volume/pages
10(1996) :5 , p. 319-324
ISSN
0890-8508
ISI
A1996VM77500001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Nucleic acid sequence-based amplification, NASBA(R), is an isothermal amplification technique for nucleic acids and was used for typing a collection of 24 Mycoplasma pneumoniae strains. A set of primers was chosen from the 16S rRNA sequence alignment of Mycoplasma species. The nucleotide sequences of the (-)RNA amplicons were determined for M. pneumoniae strains M15/83 (type 1) and FH (type 2), and revealed a one-point difference at the 16S rRNA level between the two types. Based on this result, two type-specific probes were constructed. The probes were hybridized in solution with the amplified nucleic acids of 24 M. pneumoniae strains In an enzyme linked gel assay (ELGA). The results obtained by NASBA-based typing are in agreement with the classification of the 24 M. pneumoniae strains into two types by other typing methods, confirming the reliability of this technique. (C) 1996 Academic Press Limited
E-info
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