Publication
Title
Typing of **Mycoplasma pneumoniae** by nucleic acid sequence-based amplification, NASBA®
Author
Abstract
Nucleic acid sequence-based amplification, NASBA(R), is an isothermal amplification technique for nucleic acids and was used for typing a collection of 24 Mycoplasma pneumoniae strains. A set of primers was chosen from the 16S rRNA sequence alignment of Mycoplasma species. The nucleotide sequences of the (-)RNA amplicons were determined for M. pneumoniae strains M15/83 (type 1) and FH (type 2), and revealed a one-point difference at the 16S rRNA level between the two types. Based on this result, two type-specific probes were constructed. The probes were hybridized in solution with the amplified nucleic acids of 24 M. pneumoniae strains In an enzyme linked gel assay (ELGA). The results obtained by NASBA-based typing are in agreement with the classification of the 24 M. pneumoniae strains into two types by other typing methods, confirming the reliability of this technique. (C) 1996 Academic Press Limited
Language
English
Source (journal)
Molecular and cellular probes. - London
Publication
London : 1996
ISSN
0890-8508
Volume/pages
10:5(1996), p. 319-324
ISI
A1996VM77500001
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 08.10.2008
Last edited 13.09.2017
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