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Title
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Measurement and functional analysis of the mas-related G protein-coupled receptor MRGPRX2 on human mast cells and basophils
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Author
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Abstract
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| Basophils and mast cells (MCs) are important effector cells in the immune system. For a long time, it has been known that these cells can be activated though the cross-linking of IgE antibodies bound to their high-affinity receptor (FcεRI). However, evidence has accumulated suggesting that these cells can also be activated by various IgE-independent mechanisms. Occupation of MAS Related GPR Family Member X2 (MRGPRX2), a G protein-coupled receptor, is described as an alternative IgE-independent activation mechanism. Here we describe a flow cytometric technique to analyze MRGPRX2 expression and its functionality on cultured human MCs and conditioned basophils, that is, basophils with upregulated surface expression of MRGPRX2. |
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Language
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English
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Source (journal)
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Methods in molecular biology. - Totowa, N.J., 1984, currens
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Source (book)
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Basophils and mast cells methods and protocols / Gibbs, Bernhard F. [edit.]; et al. [edit.]
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Source (series)
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Methods in molecular biology (MIMB) ; 2163
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Publication
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New York, N.Y.
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Humana
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2020
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ISSN
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1064-3745
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ISBN
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978-1-07-160695-7
978-1-07-160696-4
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DOI
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10.1007/978-1-0716-0696-4_18
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Volume/pages
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p. 219-226
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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