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Title
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Identifying the pressure points of acute cadmium stress prior to acclimation in Arabidopsis thaliana
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Author
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Abstract
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| The toxic metal cadmium (Cd) is a major soil pollutant. Knowledge on the acute Cd-induced stress response is required to better understand the triggers and sequence of events that precede plant acclimation. Therefore, we aimed to identify the pressure points of Cd stress using a short-term exposure set-up ranging from 0 h to 24 h. Acute responses related to glutathione (GSH), hydrogen peroxide (H2O2), 1-aminocyclopropane-1-carboxylic acid (ACC), ethylene and the oxidative challenge were studied at metabolite and/or transcript level in roots and leaves of Arabidopsis thaliana either exposed or not to 5 µM Cd. Cadmium rapidly induced root GSH depletion, which might serve as an alert response and modulator of H2O2 signalling. Concomitantly, a stimulation of root ACC levels was observed. Leaf responses were delayed and did not involve GSH depletion. After 24 h, a defined oxidative challenge became apparent, which was most pronounced in the leaves and concerted with a strong induction of leaf ACC synthesis. We suggest that root GSH depletion is required for a proper alert response rather than being a merely adverse effect. Furthermore, we propose that roots serve as command centre via a.o. root-derived ACC/ethylene to engage the leaves in a proper stress response. |
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Language
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English
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Source (journal)
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International journal of molecular sciences
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Publication
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2020
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ISSN
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1422-0067
1661-6596
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DOI
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10.3390/IJMS21176232
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Volume/pages
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21
:17
(2020)
, p. 1-22
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Article Reference
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6232
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ISI
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000569661800001
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Pubmed ID
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32872315
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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