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Title
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Human phase I in vitro liver metabolism of two bisphenolic diglycidyl ethers BADGE and BFDGE
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Author
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Abstract
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| Root canal sealers are commonly used to endodontically treat teeth with periapical infections. Some root canal sealers based on epoxy resin contain bisphenol A diglycidyl ether (BADGE) and bisphenol F diglycidyl ether (BFDGE). The presence of these chemicals is of concern due to the close contact to the blood stream at the apex and the long setting times of up to 24 h. These chemicals, or any of their degradation products or metabolites, can then exert their toxic effects before being excreted. This study aimed to identify the phase I in vitro biotransformation products of BADGE and BFDGE using human liver microsomes. During incubation with microsomal fractions, the epoxides were rapidly hydrolysed in a NADPH independent manner resulting in the formation of BADGE center dot 2H(2)O and BFDGE center dot 2H(2)O. Further, oxidative reactions, such as hydroxylation and carboxylation, generated other BADGE metabolites, such as BADGE center dot 2H(2)O-OH and BADGE center dot H2O center dot COOH, respectively. For BFDGE, further oxidation of BFDGE center dot 2H(2)O led to the newly reported carboxylic acid, BFDGE center dot H2O center dot COOH. In total, three specific metabolites have been identified which can serve in future human biomonitoring studies of BADGE and BFDGE. |
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Language
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English
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Source (journal)
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Toxicology letters. - Amsterdam
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Publication
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Amsterdam
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2020
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ISSN
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0378-4274
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DOI
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10.1016/J.TOXLET.2020.06.022
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Volume/pages
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332
(2020)
, p. 7-13
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ISI
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000566801900002
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Pubmed ID
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32615244
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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