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Title
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Smoking cessation and bronchial epithelial remodelling in COPD : a cross-sectional study
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Author
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Institution/Organisation
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GLUCOLD Study Grp
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Abstract
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| Background: Chronic Obstructive Pulmonary Disease ( COPD) is associated with bronchial epithelial changes, including squamous cell metaplasia and goblet cell hyperplasia. These features are partially attributed to activation of the epidermal growth factor receptor ( EGFR). Whereas smoking cessation reduces respiratory symptoms and lung function decline in COPD, inflammation persists. We determined epithelial proliferation and composition in bronchial biopsies from current and ex-smokers with COPD, and its relation to duration of smoking cessation. Methods: 114 COPD patients were studied cross-sectionally: 99 males/15 females, age 62 +/- 8 years, median 42 pack-years, no corticosteroids, current (n = 72) or ex-smokers ( n = 42, median cessation duration 3.5 years), postbronchodilator FEV 63 +/- 9% predicted. Squamous cell metaplasia (%), goblet cell ( PAS/Alcian Blue(+)) area (%), proliferating (Ki- 67(+)) cell numbers (/mm basement membrane), and EGFR expression (%) were measured in intact epithelium of bronchial biopsies. Results: Ex-smokers with COPD had significantly less epithelial squamous cell metaplasia, proliferating cell numbers, and a trend towards reduced goblet cell area than current smokers with COPD ( p = 0.025, p = 0.001, p = 0.081, respectively), but no significant difference in EGFR expression. Epithelial features were not different between short-term quitters (<3.5 years) and current smokers. Long-term quitters (= 3.5 years) had less goblet cell area than both current smokers and short-term quitters ( medians: 7.9% vs. 14.4%, p = 0.005; 7.9% vs. 13.5%, p = 0.008; respectively), and less proliferating cell numbers than current smokers (2.8% vs. 18.6%, p < 0.001). Conclusion: Ex-smokers with COPD had less bronchial epithelial remodelling than current smokers, which was only observed after long-term smoking cessation (>3.5 years). |
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Language
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English
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Source (journal)
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Respiratory research. - London
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Publication
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London
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2007
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ISSN
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1465-9921
1465-993X
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DOI
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10.1186/1465-9921-8-85
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Volume/pages
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8
(2007)
, p. 1-9
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Article Reference
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85
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ISI
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000252656900001
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Pubmed ID
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18039368
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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