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Title
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Ozone-induced inflammation assessed in sputum and bronchial, lavage fluid from asthmatics : a new noninvasive tool in epidemiologic studies on air pollution and asthma
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Author
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Abstract
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| We investigated correlations between ozone-induced increases in inflammatory markers in induced sputum and in bronchial lavage fluid. Sixteen volunteers with intermittent asthma participated in a placebo-controlled parallel study with two exposures. Six days before and 16 h after the first exposure to ozone (0.4 ppm during 2 h) sputum was induced with hypertonic saline. This resulted in a significant increase in the sputum levels of eosinophil cationic protein (ECP; 1.8-fold; p = .03), neutrophil elastase (5.0-fold; p = .005) and the total cell number (1.6-fold; p = .02). After 4 weeks, a second exposure was randomized for air or ozone. Six days before and 16 h after the second exposure a bronchial lavage was performed. ECP values in sputum and in bronchial lavage fluid obtained after ozone correlated significantly (Rs = .79; p = .04), as did interleukin-8 (IL-8) values (Rs = .86; p = .01), and the percentage eosinophils (Rs = .89; p = .007). Moreover, the ozone-induced changes in percentage eosinophils observed in sputum and lavage fluid were highly correlated (Rs = .93; p = .003). In conclusion, changes in eosinophils, IL-8, and ECP markers induced by ozone and measured in sputum reflect the inflammatory responses in the lower airways of asthmatics, and may provide a noninvasive tool in epidemiologic studies on air pollution and asthma. (C) 1999 Elsevier Science Inc. |
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Language
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English
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Source (journal)
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Free radical biology and medicine. - New York, N.Y.
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Publication
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New York, N.Y.
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1999
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ISSN
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0891-5849
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DOI
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10.1016/S0891-5849(99)00191-4
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Volume/pages
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27
:11-12
(1999)
, p. 1448-1454
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ISI
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000084334900038
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Pubmed ID
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10641740
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Full text (Publisher's DOI)
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