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Title
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Digital dipstick: miniaturized bacteria detection and digital quantification for the point-of-care
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Author
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Abstract
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| Established digital bioassay formats, digital PCR and digital ELISA, show extreme limits of detection, absolute quantification and high multiplexing capabilities. However, they often require complex instrumentation, and extensive off-chip sample preparation. In this study, we present a dipstick-format digital biosensor (digital dipstick) that detects bacteria directly from the sample liquid with a minimal number of steps: dip, culture, and count. We demonstrate the quantitative detection of Escherichia coli (E. coli) in urine in the clinically relevant range of 10(2)-10(5) CFU ml(-1) for urinary tract infections. Our format shows 89% sensitivity to detect E. coli in clinical urine samples (n = 28) when it is compared to plate culturing (gold standard). The significance and uniqueness of this diagnostic test format is that it allows a non-trained operator to detect urinary tract infections in the clinically relevant range in the home setting. |
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Language
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English
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Source (journal)
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Lab on a chip
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Publication
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Royal society of chemistry
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2020
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ISSN
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1473-0197
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DOI
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10.1039/D0LC00793E
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Volume/pages
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20
:23
(2020)
, p. 4349-4356
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ISI
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000592314900002
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Pubmed ID
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33169747
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Full text (Publisher's DOI)
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