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Title
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A novel wireless paper-based potentiometric platform for monitoring glucose in blood
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Author
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Abstract
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| A novel low-cost, compact and sensitive paper-based platform for the accurate monitoring of glucose in biological fluids is presented. Paper-based working and reference electrodes are combined to build a whole potentiometric cell, which also fits a sampling module for simple and fast determination of glucose in a single drop of blood. The working electrode is built using a platinized filter paper coated with a Nafion membrane that entraps the enzyme glucose oxidase; the reference electrode is made by casting a polyvinylbutyral-based membrane onto a conductive paper. The system works by detecting the hydrogen peroxide generated as a result of the enzymatic reaction. Selectivity is achieved due to the permselective behaviour of Nafion, while a significant enhancement of the sensitivity is reached by exploiting the Donnan-coupled formal potential. Under optimum conditions, a sensitivity of -95.9 +/- 4.8 mV per decade in the 0.3-3 mM range is obtained. Validation of the measurements has been performed against standard methods in human serum and blood. Final integration with a wireless reader allows for truly in situ measurements with a less than 2 minute procedure including a two-point calibration, washing and measurement. This low-cost analytical device opens up new prospects for rapid diagnostic results in nonlaboratory settings. |
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Language
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English
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Source (journal)
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Lab on a chip
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Publication
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Royal society of chemistry
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2017
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ISSN
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1473-0197
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DOI
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10.1039/C7LC00339K
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Volume/pages
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17
:14
(2017)
, p. 2500-2507
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ISI
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000405189000014
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Pubmed ID
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28653727
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Full text (Publisher's DOI)
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Full text (open access)
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