|
Title
|
|
|
|
Chemical and pharmacological potential of Coccoloba cowellii, an endemic endangered plant from Cuba
| |
|
Author
|
|
|
|
| |
|
Abstract
|
|
|
|
| Coccoloba cowellii Britton (Polygonaceae) is an endemic and critically endangered plant that only grows in Camagüey, a province of Cuba. In this study, a total of 13 compounds were identified in a methanolic leaf extract, employing a dereplication of the UHPLC-HRMS data by means of feature-based molecular networking (FBMN) analysis in the Global Natural Products Social Molecular Network (GNPS), together with the interpretation of the MS/MS data and comparison with the literature. The major constituents were glucuronides and glycosides of myricetin and quercetin, as well as epichatechin-3-O-gallate, catechin, epicatechin and gallic acid, all of them being reported for the first time in C. cowellii leaves. The leaf extract was also tested against various microorganisms, and it showed a strong antifungal effect against Candida albicans ATCC B59630 (azole-resistant) (IC50 2.1 g/mL) and Cryptococcus neoformans ATCC B66663 (IC50 4.1 g/mL) with no cytotoxicity (CC50 > 64.0 g/mL) on MRC-5 SV2 cells, determined by the resazurin assay. Additionally, the extract strongly inhibited COX-1 and COX-2 enzyme activity using a cell-free experiment in a dose-dependent manner, being significantly more active on COX-1 (IC50 4.9 g/mL) than on COX-2 (IC50 10.4 g/mL). The constituents identified as well as the pharmacological activities measured highlight the potential of C. cowellii leaves, increasing the interest in the implementation of conservation strategies for this species. |
| |
|
Language
|
|
|
|
English
| |
|
Source (journal)
|
|
|
|
Molecules: a journal of synthetic chemistry and natural product chemistry. - Bazel
| |
|
Publication
|
|
|
|
Bazel
:
2021
| |
|
ISSN
|
|
|
|
1420-3049
| |
|
DOI
|
|
|
|
10.3390/MOLECULES26040935
| |
|
Volume/pages
|
|
|
|
26
:4
(2021)
, 13 p.
| |
|
Article Reference
|
|
|
|
935
| |
|
ISI
|
|
|
|
000624188900001
| |
|
Pubmed ID
|
|
|
|
33578815
| |
|
Medium
|
|
|
|
E-only publicatie
| |
|
Full text (Publisher's DOI)
|
|
|
|
| |
|
Full text (open access)
|
|
|
|
| |
|