Publication
Title
The ins and outs of messenger RNA electroporation for physical gene delivery in immune cell-based therapy
Author
Abstract
Messenger RNA (mRNA) electroporation is a powerful tool for transient genetic modification of cells. This non-viral method of genetic engineering has been widely used in immunotherapy. Electroporation allows fine-tuning of transfection protocols for each cell type as well as introduction of multiple protein-coding mRNAs at once. As a pioneering group in mRNA electroporation, in this review, we provide an expert overview of the ins and outs of mRNA electroporation, discussing the different parameters involved in mRNA electroporation as well as the production of research-grade and production and application of clinical-grade mRNA for gene transfer in the context of cell-based immunotherapies.
Language
English
Source (journal)
Pharmaceutics
Publication
2021
ISSN
1999-4923
DOI
10.3390/PHARMACEUTICS13030396
Volume/pages
13 :3 (2021) , 23 p.
Article Reference
396
ISI
000634066700001
Pubmed ID
33809779
Medium
E-only publicatie
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Improved RNA-based engineering of T lymphocytes with leukemia-specific T cell receptors to redirect their effector functions: towards a clinically safe platform to evaluate efficacy and potential off-target toxicity.
Wilms' tumor (WTl) antigen-targeted dendritic cell vaccination to prevent relapse in patients with acute myeloid leukemia: a multicenter randomized phase II trial.
Maximizing the anti-tumor potency of next-generation dendritic cell vaccines by combining interleukin-15 and in situ silencing of programmed-death ligands.
Immune effector cell therapy for hematological malignancies with a focus on acute myeloid leukemia and multiple myeloma.
Safety and feasibility evaluation of first-in-human interleukin-15-transpresenting Wilms'tumor (WT1) targeting autologous dendritic cell vaccination in cancer patients.
Optimization of chimeric antigen receptor (CAR) design for improved cellular immunotherapy of hematological diseases.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 22.03.2021
Last edited 13.11.2024
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