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Title
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CD32(+)CD4(+) memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice
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Author
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Abstract
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| CD32 has raised conflicting results as a putative marker of the HIV-1 reservoir. We measured CD32 expression in tissues from viremic and virally suppressed humanized mice treated relatively early or late after HIV-1 infection with combined antiretroviral therapy. CD32 was expressed in a small fraction of the memory CD4(+) T-cell subsets from different tissues in viremic and aviremic mice, regardless of treatment initiation time. CD32(+) memory CD4(+) T cells were enriched in cell associated (CA) HIV-1 DNA but not in CA HIV-1 RNA as compared to the CD32(-) CD4(+) fraction. Using multidimensional reduction analysis, several memory CD4(+)CD32(+) T-cell clusters were identified expressing HLA-DR, TIGIT, or PD-1. Importantly, although tissue-resident CD32(+)CD4(+) memory cells were enriched with translation-competent reservoirs, most of it was detected in memory CD32-CD4(+) T cells. Our findings support that CD32 labels highly activated/exhausted memory CD4(+) T-cell subsets that contain only a small proportion of the translation-competent reservoir. |
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Language
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English
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Source (journal)
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iScience
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Publication
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Elsevier
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2021
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ISSN
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2589-0042
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DOI
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10.1016/J.ISCI.2020.101881
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Volume/pages
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24
:1
(2021)
, 33 p.
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Article Reference
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101881
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ISI
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000612996900003
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Pubmed ID
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33364576
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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