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Title
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Cytotoxic CD8+T cells expressing CXCR5 are detectable in HIV-1 elite controllers after prolonged in vitro peptide stimulation
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Author
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Institution/Organisation
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PhenoCure Study Group
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Abstract
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| Antiretroviral therapy (ART) is not curative as HIV-1 persists in long-lived viral reservoirs. Consequently, patients are dependent on life-long drug adherence with possible side effects. To overcome these limitations strategies of a functional cure aim at ART free viral remission. In this study, we sought to identify detailed subsets of anti-viral CD8(+) T cell immunity linked to natural long-term control of HIV-1 infection. Here, we analyzed HIV controllers and ART suppressed progressors for in vitro viral suppressive capacity (VSC) at baseline and after peptide stimulation. Functional properties and phenotypes of CD8(+) T cells were assessed by IFN-gamma ELISPOT and 18 color flow cytometry. HIV controllers showed significantly increased suppression at baseline as well as after peptide stimulation. IFN-gamma secretion and the proliferation marker Ki67 positively correlated with VSC. Moreover, the detailed phenotype of three distinct multifunctional memory CD8(+) T cell subsets were specific traits of HIV controllers of which two correlated convincingly with VSC. Our results underline the importance of multifunctional CD8(+) T cell responses during natural control. Especially the role of CXCR5 expressing cytotoxic subsets emphasizes potential surveillance in sites of reservoir persistence and demand further study. |
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Language
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English
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Source (journal)
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Frontiers in immunology. - Place of publication unknown
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Publication
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Place of publication unknown
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2021
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ISSN
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1664-3224
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DOI
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10.3389/FIMMU.2020.622343
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Volume/pages
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11
(2021)
, 14 p.
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Article Reference
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622343
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ISI
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000627026000001
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Pubmed ID
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33717056
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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