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Title
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Triage of human papillomavirus infected women by methylation analysis in first-void urine
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Author
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Abstract
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| Host cell DNA methylation analysis in urine provides promising triage markers for women diagnosed with a high-risk (HR) human papillomavirus (HPV) infection. In this study, we have investigated a panel of six host cell methylation markers ( GHSR, SST, ZIC1, ASCL1, LHX8, ST6GALNAC5 ) in cervicovaginal secretions collected within the first part of the urine void (FVU) from a referral population. Cytology, histology, and HPV DNA genotyping results on paired FVU and cervical samples were available. Urinary median methylation levels from HR-HPV (n = 93) positive women were found to increase for all markers with severity of underlying disease. Significantly elevated levels were observed for GHSR and LHX8 in relation to high-grade cervical intraepithelial neoplasia (CIN2 +; n = 33), with area under de curve values of 0.80 (95% Confidence Interval (CI) 0.59–0.92) and 0.76 (95% CI 0.58–0.89), respectively. These findings are the first to support the assertion that methylation analysis of host cell genes is feasible in FVU and holds promise as molecular, triage strategy to discern low- from high-grade cervical disease in HR-HPV positive women. Molecular testing on FVU may serve to increase cervical cancer screening attendance in hard-to-reach populations whilst reducing loss to follow-up and await further optimization and validation studies. |
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Language
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English
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Source (journal)
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Scientific reports. - London, 2011, currens
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Publication
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London
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Nature Publishing Group
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2021
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ISSN
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2045-2322
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DOI
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10.1038/S41598-021-87329-1
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Volume/pages
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11
:1
(2021)
, 10 p.
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Article Reference
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7862
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ISI
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000640430100003
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Pubmed ID
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33846517
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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