Assessing the receptor-mediated activity of PAHs using AhR-, ER alpha- and PPAR gamma- CALUX bioassays

Boonen, I.; Van Heyst, A.; Van Langenhove, K.; Van Hoeck, E.; Mertens, B.; Denison, M.S.; Elskens, M.; Demaegdt, H.

doi:10.1016/J.FCT.2020.111602

Title

Assessing the receptor-mediated activity of PAHs using AhR-, ER alpha- and PPAR gamma- CALUX bioassays

Author

Boonen, I.

Van Heyst, A.

Van Langenhove, K.

Van Hoeck, E.

Mertens, B.

Denison, M.S.

Elskens, M.

Demaegdt, H.

Abstract

Polycyclic aromatic hydrocarbons (PAH) are a complex group of organic compounds, consisting of at least three fused aromatic rings, which are formed during combustion of organic matter. While some PAHs have been reported to have carcinogenic and/or mutagenic properties, another possible negative health impact is their endocrine disrupting potential. Therefore, the aim of this study was to determine both the agonistic and antagonistic endocrine activity of 9 environmentally relevant PAHs using three different CALUX bioassays: The AhR-CALUX, The ER alpha-CALUX and PPAR gamma-CALUX. For the PPAR gamma-CALUX anthracene, fluoranthene, pyrene and fluorene showed weak agonistic activity, whilst benzo(a)pyrene (B(a)P) was the only one exhibiting weak antagonistic activity. For the AhR-CALUX, chrysene was the only PAH that showed relatively strong agonist activity (except for B(a)P which was used as a standard). Pyrene, anthracene and fluoranthene showed weak AhR agonist activity. In the ER alpha-CALUX bioassay, fluoranthene had agonistic activity whilst B(a)P exhibited both agonistic and antagonistic activity (lowering E2 activity by 30%). Phenanthrene and anthracene had weak ER alpha agonist activities. These results indicate that certain PAHs have multiple modes of action and can activate/inhibit multiple receptor signaling pathways known to play critical roles in mediating endocrine disruption.

Language

English

Source (journal)

Food and chemical toxicology. - Oxford

Publication

Oxford : 2020

ISSN

0278-6915

DOI

10.1016/J.FCT.2020.111602

Volume/pages

145 (2020) , 10 p.