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Title
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In vitro 50 Hz magnetic field long-term exposure : cytogenetic tests on human lymphoblastoid TK6 cells and validation of the test environment
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Author
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Abstract
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| Potential health effects of extremely low-frequency (electro)magnetic fields (ELF-(E)MFs) have long been investigated, but the results are still inconclusive. With respect to genotoxicity, sound data related to the effects of long-term exposure to ELF-(E)MFs on the genetic material and the impact of long-term pre-exposure to ELF-(E)MFs on the sensitivity of cells to the damage induced by known mutagens are needed. In this manuscript, an optimized protocol for a combined in vitro comet/micronucleus study to investigate these effects in a human lymphoblastoid cell line (TK6) is provided including the description of a well-validated exposure system. Furthermore, the use of a shielding system to limit background ELF-MFs inside the incubator is described as well. Optimized protocols for cytogenetic tests with ELF-MFs on TK6 cells ensure the reproducibility of test results. Validation of exposure environment and exposure system are needed prior to performing tests with ELF-MFs. A simple, but effective method to shield cells and reduce unintentional ELF-MF exposure consists of using the mu-metal cylinder. This is of particular interest when studying the effects of low exposure levels. (C) 2020 The Author(s). Published by Elsevier B.V. |
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Language
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English
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Source (journal)
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MethodsX
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Publication
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2020
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ISSN
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2215-0161
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DOI
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10.1016/J.MEX.2020.101071
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Volume/pages
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7
(2020)
, 16 p.
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Article Reference
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101071
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ISI
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000607660800034
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Pubmed ID
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33088726
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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