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Title
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Differentiation of gastric helicobacter species Using MALDI-TOF mass spectrometry
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Author
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Abstract
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| Gastric helicobacters (Helicobacter (H.) pylori and non-H. pylori Helicobacter species (NHPHs)) colonize the stomach of humans and/or animals. Helicobacter species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, Helicobacter species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric Helicobacter isolates of 10 different Helicobacter species using MALDI-TOF MS in order to establish a more elaborate Helicobacter reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores >= 2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related Helicobacter species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric Helicobacter species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account. |
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Language
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English
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Source (journal)
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Pathogens
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Publication
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2021
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ISSN
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2076-0817
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DOI
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10.3390/PATHOGENS10030366
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Volume/pages
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10
:3
(2021)
, 15 p.
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Article Reference
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366
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ISI
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000633977400001
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Pubmed ID
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33803832
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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