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Title
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Quantification of boron in cells for evaluation of drug agents used in boron neutron capture therapy
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Author
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Abstract
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| Boron neutron capture therapy (BNCT) is an extensively studied radiotherapeutic strategy for cancer treatment. BNCT is based on irradiation of malignant tumour cells with neutrons after uptake of a B-10 containing molecule. Alpha particles, locally produced by neutron irradiation kill the cancer cells. Important for ongoing research regarding cellular uptake and cytotoxicity of a large variety of B-10 containing molecules is the accurate determination of boron concentrations in cell cultures. In this work, the sample preparation for quantitative inductively coupled plasma mass spectrometry (ICP-MS) analysis on cell cultures was optimized. By making use of acid digestion combined with UV digestion, low detection limits (0.4 mu g L-1) and full recoveries of boron could be achieved while measurements were free of spectral and non-spectral interferences. Finally, cell-associated boron in the form of 4-borono-l-phenylalanine (l-BPA) in vascular endothelial cell cultures could be determined with ICP-MS as (1.26 +/- 0.10) x 10(9) boron atoms per cell. The developed method can prove its importance for further BNCT research and elemental analysis of cell cultures. |
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Language
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English
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Source (journal)
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Journal of analytical atomic spectrometry. - London
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Publication
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London
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2021
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ISSN
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0267-9477
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DOI
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10.1039/D0JA00456A
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Volume/pages
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36
:3
(2021)
, p. 598-606
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ISI
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000629283400009
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Full text (Publisher's DOI)
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