|
Title
|
|
|
|
End-stage cystic fibrosis lung disease is characterised by a diverse inflammatory pattern : an immunohistochemical analysis
| |
|
Author
|
|
|
|
| |
|
Abstract
|
|
|
|
| Background: Cystic fibrosis (CF) lung disease is characterised by vigorous airway inflammation eventually resulting in severe lung damage. This study aimed to describe the diversity of the inflammatory pattern in end-stage CF lungs by evaluating and quantifying which components of the innate and adaptive immunity are involved, and by assessing whether this is gender-specific. Methods: CF explant lung tissue (n = 20) collected at time of transplantation and control tissue (n = 22) was sectioned (9 mu m) and stained for neutrophils, eosinophils, mast cells, dendritic cells, macrophages, CD4 T cells, cytotoxic T cells and B cells. Quantification with special attention for immune cell location was performed. Results: Neutrophils, mast cells, dendritic cells, macrophages, CD4 T and cytotoxic T cells were significantly increased in CF compared to controls and there was a disproportionate increase of neutrophils around the airways in CF. Large amounts of lymphoid follicles were found in the CF lung and they had a skewed B cell/T cell composition. Upon subdividing the CF patients into a male and female population, eosinophils, mast cells and CD4 T cells were increased specifically in CF females. In this subpopulation, lymphoid follicles had less B cells and more CD8 T cells. Conclusion: These data demonstrate a diverse inflammatory response in the CF lung, reflected by an increase of both myeloid and lymphoid immune cells. Inflammation in the CF lung appeared to be gender-specific in our population, as the significant increase of eosinophils, mast cells and CD4 T cells was especially notable in the female subpopulation. |
| |
|
Language
|
|
|
|
English
| |
|
Source (journal)
|
|
|
|
Respiratory research. - London
| |
|
Publication
|
|
|
|
London
:
2017
| |
|
ISSN
|
|
|
|
1465-9921
1465-993X
| |
|
DOI
|
|
|
|
10.1186/S12931-016-0489-2
| |
|
Volume/pages
|
|
|
|
18
(2017)
, 9 p.
| |
|
Article Reference
|
|
|
|
10
| |
|
ISI
|
|
|
|
000391406100001
| |
|
Pubmed ID
|
|
|
|
28069067
| |
|
Medium
|
|
|
|
E-only publicatie
| |
|
Full text (Publisher's DOI)
|
|
|
|
| |
|