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Title
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Nondestructive cryomicro-CT imaging enables structural and molecular analysis of human lung tissue
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Author
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Abstract
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| Micro-computed tomography (CT) enables three-dimensional (3D) imaging of complex soft tissue structures, but current protocols used to achieve this goal preclude cellular and molecular phenotyping of the tissue. Here we describe a radiolucent cryostage that permits micro-CT imaging of unfixed frozen human lung samples at an isotropic voxel size of (11 mu m)(3) under conditions where the sample is maintained frozen at - 30 degrees C during imaging. The cryostage was tested for thermal stability to maintain samples frozen up to 8 h. This report describes the methods used to choose the materials required for cryostage construction and demonstrates that whole genome mRNA integrity and expression are not compromised by exposure to micro-CT radiation and that the tissue can be used for immunohistochemistry. The new cryostage provides a novel method enabling integration of 3D tissue structure with cellular and molecular analysis to facilitate the identification of molecular determinants of disease. |
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Language
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English
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Source (journal)
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Journal of applied physiology / American Physiological Society. - Washington, D.C.
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Publication
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Washington, D.C.
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2017
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ISSN
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8750-7587
[print]
1522-1601
[online]
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DOI
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10.1152/JAPPLPHYSIOL.00838.2016
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Volume/pages
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122
:1
(2017)
, p. 161-169
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ISI
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000393698000020
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Pubmed ID
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27856720
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Full text (Publisher's DOI)
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