Publication
Title
Plasma treatment causes structural modifications in lysozyme, and increases cytotoxicity towards cancer cells
Author
Abstract
Bacterial and mammalian proteins, such as lysozyme, are gaining increasing interest as anticancer drugs. This study aims to modify the lysozyme structure using cold atmospheric plasma to boost its cancer cell killing effect. We investigated the structure at acidic and neutral pH using various experimental techniques (circular dichroism, fluorescence, and mass spectrometry) and molecular dynamics simulations. The controlled structural modification of lysozyme at neutral pH enhances its activity, while the activity was lost at acidic pH at the same treatment conditions. Indeed, a larger number of amino acids were oxidized at acidic pH after plasma treatment, which results in a greater distortion of the lysozyme structure, whereas only limited structural changes were observed in lysozyme after plasma treatment at neutral pH. We found that the plasma-treated lysozyme significantly induced apoptosis to the cancer cells. Our results reveal that plasma-treated lysozyme could have potential as a new cancer cell killing drug.
Language
English
Source (journal)
International journal of biological macromolecules. - Guildford
Publication
Guildford : 2021
ISSN
0141-8130
DOI
10.1016/J.IJBIOMAC.2021.05.146
Volume/pages
182 (2021) , p. 1724-1736
ISI
000675794700005
Pubmed ID
34051258
Full text (Publisher's DOI)
Full text (open access)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Project info
Anticancer activity of plasma activated medium and its underlying mechanisms: Combined experimental and computational study (Anticancer-PAM).
CalcUA as central calculation facility: supporting core facilities.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 16.06.2021
Last edited 25.11.2024
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