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Title
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PapRIV, a BV-2 microglial cell activating quorum sensing peptide
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Author
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Abstract
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| Quorum sensing peptides (QSPs) are bacterial peptides produced by Gram-positive bacteria to communicate with their peers in a cell-density dependent manner. These peptides do not only act as interbacterial communication signals, but can also have effects on the host. Compelling evidence demonstrates the presence of a gut-brain axis and more specifically, the role of the gut microbiota in microglial functioning. The aim of this study is to investigate microglial activating properties of a selected QSP (PapRIV) which is produced by Bacillus cereus species. PapRIV showed in vitro activating properties of BV-2 microglia cells and was able to cross the in vitro Caco-2 cell model and reach the brain. In vivo peptide presence was also demonstrated in mouse plasma. The peptide caused induction of IL-6, TNF alpha and ROS expression and increased the fraction of ameboid BV-2 microglia cells in an NF-kappa B dependent manner. Different metabolites were identified in serum, of which the main metabolite still remained active. PapRIV is thus able to cross the gastro-intestinal tract and the blood-brain barrier and shows in vitro activating properties in BV-2 microglia cells, hereby indicating a potential role of this quorum sensing peptide in gut-brain interaction. |
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Language
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English
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Source (journal)
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Scientific reports. - London, 2011, currens
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Publication
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London
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Nature Publishing Group
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2021
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ISSN
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2045-2322
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DOI
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10.1038/S41598-021-90030-Y
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Volume/pages
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11
:1
(2021)
, 14 p.
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Article Reference
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10723
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ISI
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000658820100011
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Pubmed ID
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34021199
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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