|
Title
|
|
|
|
Plasma-derived microRNAs are influenced by acute and chronic exercise in patients with heart failure with reduced ejection fraction
| |
|
Author
|
|
|
|
| |
|
Abstract
|
|
|
|
| Background. Exercise training improves VO2peak in heart failure with reduced ejection fraction (HFrEF), but the effect is highly variable as it is dependent on peripheral adaptations. We evaluated changes in plasma-derived miRNAs by acute and chronic exercise to investigate whether these can mechanistically be involved in the variability of exercise-induced adaptations. Methods. Twenty-five male HFrEF patients (left ventricular ejection fraction <40%, New York Heart Association class ≥II) participated in a 15-week combined strength and aerobic training programme. The effect of training on plasma miRNA levels was compared to 21 male age-matched sedentary HFrEF controls. Additionally, the effect of a single acute exercise bout on plasma miRNA levels was assessed. Levels of 5 miRNAs involved in pathways relevant for exercise adaptation (miR-23a, miR-140, miR-146a, miR-191 and miR-210) were quantified using RT-qPCR and correlated with cardiopulmonary exercise test, echocardiographic, vascular function and muscle strength variables. Results. Expression levels of miR-146a decreased with training compared to controls. Acute exercise resulted in a decrease in miR-191 before, but not after training. Baseline miR-23a predicted change in VO2peak independent of age and left ventricular ejection fraction. Baseline miR-140 was independently correlated with change in load at the respiratory compensation point and change in body mass index, and baseline miR-146a with change in left ventricular mass index. Conclusions. Plasma-derived miRNAs may reflect the underlying mechanisms of exercise-induced adaptation. In HFrEF patients, baseline miR-23a predicted VO2peak response to training. Several miRNAs were influenced by acute or repeated exercise. These findings warrant exploration in larger patient populations and further mechanistic in-vitro studies on their molecular involvement. |
| |
|
Language
|
|
|
|
English
| |
|
Source (journal)
|
|
|
|
Frontiers in physiology / Frontiers Research Foundation (Lausanne, Switzerland) - [Lausanne], 2010, currens
| |
|
Publication
|
|
|
|
[Lausanne]
:
Frontiers Research Foundation
,
2021
| |
|
ISSN
|
|
|
|
1664-042X
| |
|
DOI
|
|
|
|
10.3389/FPHYS.2021.736494
| |
|
Volume/pages
|
|
|
|
12
(2021)
, 12 p.
| |
|
Article Reference
|
|
|
|
736494
| |
|
ISI
|
|
|
|
000717299000001
| |
|
Pubmed ID
|
|
|
|
34646160
| |
|
Medium
|
|
|
|
E-only publicatie
| |
|
Full text (Publisher's DOI)
|
|
|
|
| |
|
Full text (open access)
|
|
|
|
| |
|