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Title
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Safety testing of an antisense oligonucleotide intended for pediatric indications in the juvenile Gottingen Minipig, including an evaluation of the ontogeny of key nucleases
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Author
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Abstract
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| The adult Gottingen Minipig is an acknowledged model for safety assessment of antisense oligonucleotide (ASO) drugs developed for adult indications. To assess whether the juvenile Gottingen Minipig is also a suitable nonclinical model for pediatric safety assessment of ASOs, we performed an 8-week repeat-dose toxicity study in different age groups of minipigs ranging from 1 to 50 days of age. The animals received a weekly dose of a phosphorothioated locked-nucleic-acid-based ASO that was assessed previously for toxicity in adult minipigs. The endpoints included toxicokinetic parameters, in-life monitoring, clinical pathology, and histopathology. Additionally, the ontogeny of key nucleases involved in ASO metabolism and pharmacologic activity was investigated using quantitative polymerase chain reaction and nuclease activity assays. Similar clinical chemistry and toxicity findings were observed; however, differences in plasma and tissue exposures as well as pharmacologic activity were seen in the juvenile minipigs when compared with the adult data. The ontogeny study revealed a differential nuclease expression and activity, which could affect the metabolic pathway and pharmacologic effect of ASOs in different tissues and age groups. These data indicate that the juvenile Gottingen Minipig is a promising nonclinical model for safety assessment of ASOs intended to treat disease in the human pediatric population. |
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Language
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English
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Source (journal)
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Pharmaceutics
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Publication
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2021
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ISSN
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1999-4923
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DOI
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10.3390/PHARMACEUTICS13091442
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Volume/pages
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13
:9
(2021)
, 33 p.
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Article Reference
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1442
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ISI
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000700141600001
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Pubmed ID
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34575518
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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