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Title
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Embryonic progenitor pools generate diversity in fine-scale excitatory cortical subnetworks
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Author
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Abstract
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| The mammalian neocortex is characterized by a variety of neuronal cell types and precise arrangements of synaptic connections, but the processes that generate this diversity are poorly understood. Here we examine how a pool of embryonic progenitor cells consisting of apical intermediate progenitors (aIPs) contribute to diversity within the upper layers of mouse cortex. In utero labeling combined with single-cell RNA-sequencing reveals that aIPs can generate transcriptionally defined glutamatergic cell types, when compared to neighboring neurons born from other embryonic progenitor pools. Whilst sharing layer-associated morphological and functional properties, simultaneous patch clamp recordings and optogenetic studies reveal that aIP-derived neurons exhibit systematic biases in both their intralaminar monosynaptic connectivity and the post-synaptic partners that they target within deeper layers of cortex. Multiple cortical progenitor pools therefore represent an important factor in establishing diversity amongst local and long-range fine-scale glutamatergic connectivity, which generates subnetworks for routing excitatory synaptic information. |
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Language
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English
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Source (journal)
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Nature communications
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Publication
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2019
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ISSN
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2041-1723
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DOI
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10.1038/S41467-019-13206-1
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Volume/pages
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10
(2019)
, 16 p.
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Article Reference
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5224
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ISI
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000497691000006
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Pubmed ID
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31745093
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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