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Title
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Generation of vessel co-option lung metastases mouse models for single-cell isolation of metastases-derived cells and endothelial cells
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Author
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Abstract
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| Tumor vessel co-option, a process in which cancer cells "hijack"pre-existing blood vessels to grow and invade healthy tissue, is poorly understood but is a proposed resistance mechanism against anti-angiogenic therapy (AAT). Here, we describe protocols for establishing murine renal (RENCA) and breast (4T1) cancer lung vessel co-option metastases models. Moreover, we outline a reproducible protocol for single-cell isolation from murine lung metastases using magnetic-activated cell sorting as well as immunohistochemical stainings to distinguish vessel co-option from angiogenesis.For complete details on the use and execution of this protocol, please refer to Teuwen et al. (2021). |
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Language
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English
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Source (journal)
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STAR protocols
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Publication
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2022
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DOI
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10.1016/J.XPRO.2022.101691
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Volume/pages
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3
:4
(2022)
, p. 1-21
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Article Reference
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101691
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ISI
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001059380200007
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Pubmed ID
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36173713
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Medium
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E-only publicatie
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Full text (Publisher's DOI)
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Full text (open access)
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