Publication
Title
Generation of vessel co-option lung metastases mouse models for single-cell isolation of metastases-derived cells and endothelial cells
Author
Abstract
Tumor vessel co-option, a process in which cancer cells "hijack"pre-existing blood vessels to grow and invade healthy tissue, is poorly understood but is a proposed resistance mechanism against anti-angiogenic therapy (AAT). Here, we describe protocols for establishing murine renal (RENCA) and breast (4T1) cancer lung vessel co-option metastases models. Moreover, we outline a reproducible protocol for single-cell isolation from murine lung metastases using magnetic-activated cell sorting as well as immunohistochemical stainings to distinguish vessel co-option from angiogenesis.For complete details on the use and execution of this protocol, please refer to Teuwen et al. (2021).
Language
English
Source (journal)
STAR protocols
Publication
2022
DOI
10.1016/J.XPRO.2022.101691
Volume/pages
3 :4 (2022) , p. 1-21
Article Reference
101691
ISI
001059380200007
Pubmed ID
36173713
Medium
E-only publicatie
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identifier
Creation 02.10.2023
Last edited 24.05.2024
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