Publication
Title
Activation of TGFβ1 signaling enhances early dopaminergic differentiation in unrestricted somatic stem cells
Author
Abstract
So far there is increasing evidence for the involvement of transforming growth factor beta TGFβ (transforming growth factor) in differentiation and maintenance of midbrain dopaminergic neurons. Considering that USSCs (unrestricted somatic stem cells) have the potentials to differentiate into neuron-like cells and even dopaminergic neurons and that no evidence available on the role of TGFβ signaling in dopaminergic differentiation of these cells, we investigated the presence of TGFβ signaling components in USSCs and their involvement on USSCs differentiation into early dopaminergic neurons. Our results showed that components of TGFβ signaling were present and functional in undifferentiated USSCs, after which the neurally induced USSCs treated with TGFβ1 for 3 days resulted in increased expression of β-tubulin III (a general neuronal marker) and Nurr-1 (an early dopaminergic marker) at both mRNA and protein levels. Consistently, TGFβ inhibition in culture medium by using SB431542 in the presence or absence of TGFβ1, significantly decreased the expression of both neural markers. We therefore suggest that activation of TGFβ signaling-pathway in neurally induced USSCs enhances neural differentiation with an early dopaminergic phenotype which points at the positive role of the TGFβ signaling pathway toward dopaminergic fate.
Language
English
Source (journal)
Neuroscience letters. - Amsterdam
Publication
Amsterdam : 2014
ISSN
0304-3940
DOI
10.1016/J.NEULET.2014.08.055
Volume/pages
583 (2014) , p. 60-64
ISI
000345604300012
Pubmed ID
25233862
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identifier
Creation 02.10.2023
Last edited 17.08.2024
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