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Title
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Diagnosing viral infections through T cell receptor sequencing of activated CD8+ T cells
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Author
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Abstract
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| T cell-based diagnostic tools identify pathogen exposure, but lack differentiation between recent and historic exposures in acute infectious diseases. Here, T cell receptor (TCR) RNA sequencing was performed on HLADR+/CD38+ CD8+ T cell subsets of hospitalized COVID-19 patients (n = 30) and healthy controls (n = 30; ten of whom had previously been exposed to SARS-CoV-2). CDR3α and CDR3β TCR regions were clustered separately before epitope specificity annotation using a database of SARS-CoV-2 associated CDR3α and CDR3β sequences corresponding to >1000 SARS-CoV-2 epitopes. The depth of the SARS-CoV-2 associated CDR3α/β sequences differentiated COVID-19 patients from the healthy controls with a receiver operating characteristic curve (ROC) area under the curve (AUC) of 0.84 ± 0.10. Hence, annotating TCR sequences of activated CD8+ T cells can be used to diagnose an acute viral infection and discriminate it from historic exposure. In essence, this work presents a new paradigm of applying the T cell repertoire to accomplish TCR-based diagnostics. |
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Language
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English
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Source (journal)
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The journal of infectious diseases. - Chicago, Ill.
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Publication
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Chicago, Ill.
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2024
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ISSN
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0022-1899
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DOI
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10.1093/INFDIS/JIAD430
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Volume/pages
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229
:2
(2024)
, p. 507-516
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ISI
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001086250000001
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Pubmed ID
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37787611
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Full text (Publisher's DOI)
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Full text (open access)
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