Publication
Title
An internal promoter drives the expression of a truncated form of CCC1 capable of protecting yeast from iron toxicity
Author
Abstract
In yeast, iron storage and detoxification depend on the Ccc1 transporter that mediates iron accumulation in vacuoles. While deletion of the CCC1 gene renders cells unable to survive under iron overload conditions, the deletion of its previously identified regulators only partially affects survival, indicating that the mechanisms controlling iron storage and detoxification in yeast are still far from well understood. This work reveals that CCC1 is equipped with a complex transcriptional structure comprising several regulatory regions. One of these is located inside the coding sequence of the gene and drives the expression of a short transcript encoding an N-terminally truncated protein, designated as s-Ccc1. s-Ccc1, though less efficiently than Ccc1, is able to promote metal accumulation in the vacuole, protecting cells against iron toxicity. While the expression of the s-Ccc1 appears to be repressed in the normal genomic context, our current data clearly demonstrates that it is functional and has the capacity to play a role under iron overload conditions.
Language
English
Source (journal)
Microorganisms
Publication
2021
ISSN
2076-2607
DOI
10.3390/MICROORGANISMS9061337
Volume/pages
9 :6 (2021) , p. 1-14
ISI
000665930700001
Pubmed ID
34203091
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Research group
Publication type
Subject
External links
Web of Science
Record
Identifier
Creation 20.10.2023
Last edited 13.04.2024
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