Publication
Title
A GFP promoter fusion library for the study of Salmonella biofilm formation and the mode of action of biofilm inhibitors
Author
Abstract
Salmonella, an important foodborne pathogen, forms biofilms in many different environments. The composition of these biofilms differs depending on the growth conditions, and their development is highly coordinated in time. To develop efficient treatments, it is therefore essential that biofilm formation and its inhibition be understood in different environments and in a time-dependent manner. Many currently used techniques, such as transcriptomics or proteomics, are still expensive and thus limited in their application. Therefore, a GFP-promoter fusion library with 79 important Salmonella biofilm genes was developed (covering among other things matrix production, fimbriae and flagella synthesis, and c-di-GMP regulation). This library is a fast, inexpensive, and easy-to-use tool, and can therefore be conducted in different experimental setups in a time-dependent manner. In this paper, four possible applications are highlighted to illustrate and validate the use of this reporter fusion library.
Language
English
Source (journal)
Biofouling. - London
Publication
Abingdon : Taylor & francis ltd , 2014
ISSN
0892-7014
DOI
10.1080/08927014.2014.907401
Volume/pages
30 :5 (2014) , p. 605-625
ISI
000335849800008
Pubmed ID
24735176
Full text (Publisher's DOI)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identifier
Creation 31.10.2023
Last edited 01.11.2023
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