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Title
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Plant cell wall patterning and expansion mediated by protein-peptide-polysaccharide interaction
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Author
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Abstract
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| Assembly of cell wall polysaccharides into specific patterns is required for plant growth. A complex of RAPID ALKALINIZATION FACTOR 4 (RALF4) and its cell wall–anchored LEUCINE-RICH REPEAT EXTENSIN 8 (LRX8)–interacting protein is crucial for cell wall integrity during pollen tube growth, but its molecular connection with the cell wall is unknown. Here, we show that LRX8-RALF4 complexes adopt a heterotetrametric configuration in vivo, displaying a dendritic distribution. The LRX8-RALF4 complex specifically interacts with demethylesterified pectins in a charge-dependent manner through RALF4’s polycationic surface. The LRX8-RALF4-pectin interaction exerts a condensing effect, patterning the cell wall’s polymers into a reticulated network essential for wall integrity and expansion. Our work uncovers a dual structural and signaling role for RALF4 in pollen tube growth and in the assembly of complex extracellular polymers. |
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Language
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English
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Source (journal)
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Science / American Association for the Advancement of Science [Washington, D.C.] - Washington, D.C., 1880, currens
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Publication
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Washington, D.C.
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American Association for the Advancement of Science
,
2023
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ISSN
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0036-8075
[print]
1095-9203
[online]
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DOI
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10.1126/SCIENCE.ADI4720
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Volume/pages
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382
:6671
(2023)
, p. 719-725
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ISI
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001138596500040
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Pubmed ID
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37943924
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Full text (Publisher's DOI)
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Full text (open access)
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Full text (publisher's version - intranet only)
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