Publication
Title
Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers
Author
Abstract
The presence of SARS-CoV-2 in raw wastewaters has been demonstrated in many countries affected by this pandemic. Nevertheless, virus presence and infectivity in treated wastewaters, but also in the receiving water bodies are still poorly investigated. In this study, raw and treated samples from three wastewater treatment plants, and three river samples within the Milano Metropolitan Area, Italy, were surveyed for SARS-CoV-2 RNA detection by means of real time RT-PCR and infectivity test on culture cells. SARS-CoV-2 RNA was detected in raw, but not in treated wastewaters (four and two samples, respectively, sampled in two dates). The isolated virus genome was sequenced, and belonged to the strain most spread in Europe and similar to another found in the same region. RNA presence in raw wastewater samples decreased after eight days, probably following the epidemiological trend estimated for the area. Virus infectivity was always null, indicating the natural decay of viral pathogenicity in time from emission. Samples from receiving rivers (three sites, sampled in the same dates as wastewaters) showed in some cases a positivity to real time RT-PCR, probably due to non-treated, or inefficiently treated discharges, or to the combined sewage overflows. Nevertheless, also for rivers infectivity was null. Risks for public health should be limited, although a precautionary approach to risk assessment is here advocated, giving the preliminary nature of the presented data. (C) 2020 Elsevier B.V. All rights reserved.
Language
English
Source (journal)
The science of the total environment. - Amsterdam, 1972, currens
Publication
Amsterdam : 2020
ISSN
0048-9697 [print]
1879-1026 [online]
DOI
10.1016/J.SCITOTENV.2020.140911
Volume/pages
744 (2020) , 8 p.
Article Reference
140911
ISI
000573549300015
Pubmed ID
32693284
Full text (Publisher's DOI)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identifier
Creation 27.11.2023
Last edited 29.11.2023
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