Publication
Title
A multifaceted approach for evaluating Hepatitis E virus infectivity in vitro : cell culture and innovative molecular methods for integrity assessment
Author
Abstract
Hepatitis E virus is a prominent cause of viral hepatitis worldwide. In Western countries, most infections are asymptomatic. However, acute self-limiting hepatitis and chronic cases in immunocompromised individuals can occur. Studying HEV is challenging due to its difficulty to grow in cell culture. Consequently, the detection of the virus mainly relies on RT-qPCR, which cannot differentiate between infectious and non-infectious particles. To overcome this problem, methods assessing viral integrity offer a possible solution to differentiate between intact and damaged viruses. This study aims at optimizing existing HEV cell culture models and RT-qPCR-based assays for selectively detecting intact virions to establish a reliable model for assessing HEV infectivity. In conclusion, these newly developed methods hold promise for enhancing food safety by identifying approaches for inactivating HEV in food processing, thereby increasing food safety measures.
Language
English
Source (journal)
Veterinary sciences
Publication
2023
ISSN
2306-7381
DOI
10.3390/VETSCI10120676
Volume/pages
10 :12 (2023) , p. 1-20
Article Reference
676
ISI
001131995700001
Pubmed ID
38133227
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Project info
Hepatitis B-specific B cells: Rehabilitating the B in HBV.
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 28.11.2023
Last edited 01.02.2024
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