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Title
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Addition of bone-marrow mesenchymal stem cells to 3D-printed alginate/gelatin hydrogel containing freeze-dried bone nanoparticles accelerates regeneration of critical size bone defects
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Author
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Abstract
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| A 3D-printed biodegradable hydrogel, consisting of alginate, gelatin, and freeze-dried bone allograft nanoparticles (npFDBA), is developed as a scaffold for enhancing cell adhesion, proliferation, and osteogenic differentiation when combined with rat bone marrow mesenchymal stem cells (rBMSCs). This composite hydrogel is intended for the regeneration of critical-sized bone defects using a rat calvaria defect model. The behavior of rBMSCs seeded onto the scaffold is evaluated through scanning electron microscope, MTT assays, and quantitative real-time PCR. In a randomized study, thirty rats are assigned to five treatment groups: 1) rBMSCs-loaded hydrogel, 2) rBMSCs-loaded FDBA microparticles, 3) hydrogel alone, 4) FDBA alone, and 5) an empty defect serving as a negative control. After 8 weeks, bone regeneration is assessed using H&E, Masson's trichrome staining, and immunohistochemistry. The 3D-printed hydrogel displays excellent adhesion, proliferation, and differentiation of rBMSCs. The rBMSCs-loaded hydrogel exhibits comparable new bone regeneration to the rBMSCs-loaded FDBA group, outperforming other groups with statistical significance (P-value < 0.05). These findings are corroborated by Masson's trichrome staining and osteocalcin expression. The rBMSCs-loaded 3D-printed hydrogel demonstrates promising potential for significantly enhancing bone regeneration, surpassing the conventional clinical approach (FDBA). |
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Language
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English
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Source (journal)
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Macromolecular bioscience. - Weinheim, 2001 - 2012
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Publication
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Weinheim
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Wiley-v c h verlag gmbh
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2024
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ISSN
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1616-5187
[print]
1616-5195
[online]
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DOI
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10.1002/MABI.202300065
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Volume/pages
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24
:3
(2024)
, p. 1-16
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Article Reference
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2300065
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ISI
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001095926900001
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Pubmed ID
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37846197
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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